北京大学学报（医学版）2013，Vol.45Issue(3)：412-416,5.DOI:10.3969/j.issn.1671-167X.2013.03.014
纳米银材料中可溶性银离子对皮肤细胞间隙连接通讯的影响
Effects of silver ion of silver nanoparticles on gap junctional intercellular communication of human skin cells
摘要
Abstract
Objective:To further investigate the effects of silver ion (Ag +) dissolved from silver nanoparticles (AgNps) on gap junctional intercellular communication (GJIC) between HaCaT cells.Methods:In this study,20 000 × g of 1 g/L AgNps suspension were centrifuged at 4 C for 2 h,then the supernatant was collected as Ag+ stock solution and measured by inductively coupled plasma massspectrometry (ICP-MS) ; GJIC was detected by the scrape loading/dye transfer assay; connexin 43(Cx43) protein and mRNA level were estimated by Western-blot and RT-PCR,respectively.Results:Different concentrations of Ag+ (0.01,0.1 and 1.0 mg/L) did not affect the GJIC significantly.No notable changes were observed in expression of Cx43 protein and mRNA.Conclusion:The characteristics of Ag+ and the effects on gap junctional intercellular communication between HaCaT cells may be different from those of AgNps.关键词
金属纳米粒子/银/细胞间通讯/连接蛋白43/缝隙接合部Key words
Metal nanoparticles/ Silver/ Cell communication/ Connexin 43/ Gap junctions分类
医药卫生引用本文复制引用
秦宇,邓芙蓉,魏红英,韩丽敏,许珺辉,郭新彪..纳米银材料中可溶性银离子对皮肤细胞间隙连接通讯的影响[J].北京大学学报（医学版）,2013,45(3):412-416,5.基金项目
国家自然科学基金(21077006)和国家高技术研究发展计划(863计划)(2012AA062804)资助Supported by the National Natural Science Foundation of China (21077006) and the National High Technology Research and Development Program of China (863 Program)(2012AA062804) （21077006）
