北京大学学报（医学版）2013，Vol.45Issue(3)：452-457,6.DOI:10.3969/j.issn.1671-167X.2013.03.022
半乳糖凝集素在脐带间充质干细胞中的表达
Expression of Galectins in umbilical cord mesenchymal stem cells
摘要
Abstract
Objective:To investigate the expression of Galectins in umbilical cord mesenchymal stem cells (UC-MSCs) from Wharton' s jelly.Methods:Umbilical cords were obtained sterilely from full term caesarean infants,then mesenchymal stem cells (MSCs) were isolated from Wharton' s jelly of the umbilical cord via tissue cultivation.The morphology of UC-MSCs was observed under the optical microscope,and its immunophenotypes were analyzed by flow cytometry.The differentiation of UC-MSCs into the osteoblasts and adipocytes was determined utilizing yon Kossa calcium node staining and oil red O staining,respectively.The expression of Galectins at mRNA level was measured by RT-PCR.The levels of secretory Galectin-3 in culture supernatants were detected by sandwich enzyme-linked immunosorbent assay.Results:The UC-MSCs could be generated by tissue cultivation.Flow cytometry showed they highly expressed membrane molecules,such as CD29,CD44,CD73,CD90 and CD105,but did not express hematopoietic specific markers (CD14,CD34,and CD45) and immune rejection related molecule HLA-DR.UC-MSCs could differentiate into osteoblasts or adipocytes under appropriate experimental conditions.At the mRNA level,Galectin-1,3,4,8 and 9 were detected in UC-MSCs.And they also could secrete soluble Galectin-3 in a cell number dependent manner.Statistical differences were obtained among the different cell number incubation groups(F =16.901,P =0.002).However,the secretory manner of Galectin-3 was not time dependent.Conclusion:UC-MSCs,derived from Wharton' s jelly,were successfully cultured via tissue cultivation,and they could express secretory Galectin-3.All these data laid the foundation for further detecting the immunomodulatory mechanism of UC-MSCs.关键词
半乳糖凝集素类/间充质干细胞/脐带/基因表达Key words
Galectins/ Mesenchymal stem cells/ Umbilical cord/ Gene expression分类
医药卫生引用本文复制引用
李常虹,孙琳,张颖健,赵金霞,姚中强,徐宁,刘蕊,刘湘源..半乳糖凝集素在脐带间充质干细胞中的表达[J].北京大学学报（医学版）,2013,45(3):452-457,6.基金项目
国家自然科学基金(81072474,81273293)和北京市自然科学基金(7112143)资助Supported by National Natural Science Foundation of China(81072474,81273293) and Beijing Municipal Natural Science Foundation(7112143) （81072474,81273293）
