草业学报2013，Vol.22Issue(3)：132-138,7.DOI:10.11686/cyxb20130317
药用植物柽柳内生真菌分离及其抑菌活性初步研究
Isolation of endophytic fungi from the medicinal plant Tamarix chinensis and their microbial inhibition activity
摘要
Abstract
To explore the resource diversity and microbial inhibition activity of endophytic fungi from the medicinal plant Tamarix chinensis,endophytic fungi were isolated from root,stem,and leaf of the host by tissue inoculation culture.Five pathogenic fungi and four bacterial strains were used as indicators to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion.Eighteen endophytic fungal strains were isolated:most from the stems and least from the leaves.The isolated strains were divided into seven genera,three families,and two orders based on morphological characteristics.Among the isolates,eleven strains (61.1％ of the total isolated) were found to have some microbial inhibition activities against two or more pathogenic fungi,eight strains (44.4％) had evident inhibition activity against Staphylococcus aureus with strain TCEFL001 showing evident antifungal and antibacterial activities against Colletotrichum gloeosporioides,Gaeumannomyces graminis var.tritici and S.aureus,belonging to Phacodium sp.The medicinal plant Tamarix chinensis is a good endophytic fungal resource,which is worth further study for its development and application.关键词
药用植物/柽柳/内生真菌/分离/抑菌活性Key words
medicinal plant/ Tamarix chinensis/ endophytic fungus/ isolation/ microbial inhibition activity分类
农业科技引用本文复制引用
毕江涛,马萍,杨志伟,关晓庆..药用植物柽柳内生真菌分离及其抑菌活性初步研究[J].草业学报,2013,22(3):132-138,7.基金项目
宁夏银川市科技攻关项目(银财2010452)资助. （银财2010452）
