草业学报2013，Vol.22Issue(3)：321-326,6.DOI:10.11686/cyxb20130341
生防木霉对草坪土壤微生物区系的影响及定殖能力研究
Impact and colonisation ability of Trichoderma biocontrol on lawn soil microflora
摘要
Abstract
To screen for a selective medium suitable for cultivation of bacteria,fungi and actinomycetes in the soil,19 types of medium were tested.Beef extract peptone medium +Jia Shuang Shuang Mei,Mading medium+ 1 mL 1％ rose bengal and Martin medium+0.1 g potassium dichromate were best for culturing bacteria,fungi and actinomycetes.The numbers of bacteria,fungi and actinomycetes were counted after Trichoderma aureoviride T2 strain was applied to the lawn soil.T.aureoviride cause significant changes in the microbial community structure.Seventeen days after T.aureoviride was applied,the number of fungi decreased by 4 001 cfu/g but the number of bacteria increased by 1.27 × 1010 cfu/g compared with the control,while the number of actinomyces did not change significantly.The ability of T.aureoviride colonisation demonstrated that T.aureoviride could colonise the soil of turf grass within one month.Twenty five days after T.aureoviride was applied,the relative content of T.aureoviride was the lowest,but 5 days later it increased and then remained stable.关键词
深绿木霉/真菌/细菌/放线菌/定殖Key words
Trichoderma aureoviride/ fungus/ bacterium/ actinomycete/ colonisation分类
农业科技引用本文复制引用
古丽君,徐秉良,梁巧兰,尹婷..生防木霉对草坪土壤微生物区系的影响及定殖能力研究[J].草业学报,2013,22(3):321-326,6.基金项目
草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-013),甘肃省农牧厅生物技术专项(GNSW-2009-04)和甘肃省教育厅项目资助. （CY-GG-2006-013）
