动物学研究2013，Vol.34Issue(4)：399-405,7.DOI:10.11813/j.issn.0254-5853.2013.4.0399
香鱼(Plecoglossus altivelis)NFκB抑制因子α基因paIкBa克隆及表达
Cloning and expression analysis of the NFκB inhibitor IκBα of ayu (Plecoglossus altivelis)
摘要
Abstract
The NFκB inhibitor (IκBα) is an integral part of NFκB/IκB signaling pathways,which plays roles in a variety ot immune responses,such as bacterial infection resistance.By interacting with nuclear transcription factor NFκ B,IκBαt controls a variety of biological immune gene expressions.In this study,full-length cDNA (1341 bp) of the NFκ B inhibitor IκBα (PaIκ Bα,GenBank Accession No.JN801027) of Plecoglossus altivelis was obtained by RACE and PCR,and included a 5' untranslated region (UTR)(64 bp),a 3' untranslated region (UTR) (341 bp) and an open reading frame (ORF) (936 bp) encoding a polypeptide of 311 amino acids.PaIκBα had high homology with other IκBαs,containing a conserved ankyrin repeat domain,which was required for interacting with NFκB,a PEST sequence in the C-terminus and a signal responsive domain in the N-terminus.The deduced amino acid sequence of PaIκ Bα shared 95％ homology with Osmerus mordax,and 76％,75％,70％,and 68％ homology with Salmo salar,Oncorhynchus mykiss,Nile tilapia,and Siniperca chuatsi,respectively.Phylogenetic analysis revealed that Iκ Bαt of ayu and Osmerus mordax,Salmo salar,Oncorhynchus mykiss,Nile tilapia,and Siniperca chuatsi were in the same phylogenetic tree.RT-PCR analysis showed that PaIκBα mRNA expression was highest in the liver,kidney,intestine,and gills,then followed by the spleen,brain and muscle,and was lowly expressed in the heart.Likewise,afierAeromonas hydrophila infection,the mRNA level ofayu PaIκ Bα in the liver was also up-regulated.关键词
香鱼/IκBα/克隆/嗜水气单胞菌/基因表达Key words
Plecoglossus altivelis/ IκBα/ Clone/ Aeromonas hydrophila/ Gene expression分类
生物科学引用本文复制引用
张文青,龚一富,章丽,李军,刘晓丹..香鱼(Plecoglossus altivelis)NFκB抑制因子α基因paIкBa克隆及表达[J].动物学研究,2013,34(4):399-405,7.基金项目
浙江省海洋生物技术产业创新团队项目(2012R10029 （）
2012R10029-07) （）
宁波市科技厅项目(2010C91050 （）
2010C10051 （）
2010C10057) （）
宁波大学学科项目(XKL121和XKL11D2099) （XKL121和XKL11D2099）
