南方医科大学学报2013，Vol.33Issue(7)：967-971,5.DOI:10.3969/j.issn.1673-4254.2013.07.06
低硒大鼠心肌线粒体STAT3的活性变化及其与心肌损伤的关系
Change of cardiac mitochondrial STAT3 activity in rats with selenium deficiency and its relation with myocardial injury
摘要
Abstract
Objective To study the phosphorylation activity of mitochondrial signal transducer and activator of transcription 3 (STAT3) in the myocardium of rats with selenium deficiency and its association with myocardial injury.Methods Thirty-six rats were randomized into normal control group (n=18) and selenium deficiency model group (n=18) for feeding with normal and low-selenium chow,respectively,for 20,30 and 40 weeks.The cardiac function of the rats was evaluated by carotid artery intubation,and the damage of cardiac mitochondria was observed under electron microscopy.The cardiac mitochondria were extracted for assessing succinate dehydrogenase and cytochrome C oxidase activities,and the protein expressions of phosphorylated and total STAT3 were detected.Results Compared with the corresponding control groups,the rats in the model group showed significantly decreased cardiac function with obvious structural and functional damage of the cardiac mitochondria (P＜0.05),which aggravated as the low-selenium feeding time extended (P＜0.05).The rats in the model group also showed significantly decreased mitochondrial STAT3 activity (p-STAT3/STAT3) in the myocardium as the low-selenium feeding time prolonged (P＜0.05).Pearson linear correlation analysis showed that the activity of cardiac mitochondrial STAT3 had positive correlations with the left ventricular systolic pressure,maximal increased rate of the left ventricular pressure,and the activities of succinate dehydrogenase and cytochrome C oxidase (P＜0.01).Conclusion Selenium deficiency down-regulates the activity of mitochondrial STAT3 in rat heart to contribute to cardiac mitochondrial injury and the progression of heart failure.关键词
低硒/心肌线粒体/信号转导和转录激活子3/心功能Key words
low selenium/ cardiac mitochondria/ signal transducer and activator of transcription 3/ cardiac function引用本文复制引用
张明,魏瑾,潘晓青,单虎,闫蕊,薛嘉虹,朱延河,林琳..低硒大鼠心肌线粒体STAT3的活性变化及其与心肌损伤的关系[J].南方医科大学学报,2013,33(7):967-971,5.基金项目
国家自然科学基金(30771862,81170209,81100210) （30771862,81170209,81100210）
陕西省科技攻关项目(2007K13-03)Supported by National Natural Science Foundation of China (30771862,81170209,81100210). （2007K13-03）
