南方医科大学学报2013，Vol.33Issue(7)：977-982,6.DOI:10.3969/j.issn.1673-4254.2013.07.08
骨形态发生蛋白-2、-3对胚胎小鼠椎间盘细胞的成软骨及成骨作用
Effect of BMP-2 and-3 overexpression on osteogenic and chondrogenic differentiation of prenatal mouse intervertabral disc cells in vitro
摘要
Abstract
Objective To investigate the effect of recombinant adenovirus-mediated bone morphogenetic protein (BMP)-2 and-3 overexpressions on chondrogenesis and osteogenesis of prenatal mouse intervertabral disc cells and provide experimental evidences for the application of BMPs in the therapy of disc diseases.Methods The prenatal mouse intervertabral disc cells were infected with a recombinant adenovirus expressing BMP-2 and BMP-3 for 5-7 days,and the expressions of collagen type Ⅰ (Col Ⅰ),collagen type Ⅱ (Col Ⅱ),aggrecan,osteocalcin,osteoprotegerin and osteopontin mRNAs were detected with RT-PCR.The expression of cartilage matrix was evaluated with toluidine blue staining,and alkaline phosphatase (ALP) activity was detected with ALP reading and ALP staining.Results BMP-2 and-3 overexpression did not enhance chondrogenesis and osteogenesis of annulus fibrosus (AF) cells or cause significant increases in the expressions of Col Ⅰ,Col Ⅱ or aggrecan mRNA in nucleus pulposus (NP) cells.Adenovirus-mediated overexpression of BMP-2 and BMP-3,however,promoted osteogenesis of NP cells and significantly increased the expression of osteocalcin mRNA; the overexpression of BMP-2,but not BMP-3,enhanced the mRNA expressions of osteoprotegerin and osteopontin.Toluidine blue staining demonstrated that BMP-2 and BMP-3 overexpression did not obviously affect the secretion of cartilage matrix.In NP cells,BMP-2 and-3 overexpression significantly enhanced ALP activity,which was not observed in AF cells.Conclusion Adenovirus-mediated BMP-2 and-3 overexpression can promote the osteogenic differentiation of NP cells but can not affect osteogenesis of AF cells or chondrogenesis of NP cells.关键词
骨形态发生蛋白/胚胎/成骨/成软骨/椎间盘细胞Key words
bone morphogenetic protein/ prenatal/ osteogenesis/ chondrogenesis/ intervertebral disc cells引用本文复制引用
周建武,何通川,毕杨,刘传康,宿玉玺..骨形态发生蛋白-2、-3对胚胎小鼠椎间盘细胞的成软骨及成骨作用[J].南方医科大学学报,2013,33(7):977-982,6.基金项目
国家自然科学基金(81001197)Supported by National Natural Science Foundation of China (81001197). （81001197）
