南方医科大学学报2013，Vol.33Issue(7)：990-993,4.DOI:10.3969/j.issn.1673-4254.2013.07.10
胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响
Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein
摘要
Abstract
Objective To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium.Methods SD rats received intraperitoneal injections of gastrin,omeprazole or physiological saline.The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry,and its mRNA levels determined by RT-PCR.The serum calcium and phosphate levels in the rats were also detected.Results Immunohistochemistry showed that 1,25D3-MARRS binding protein was expressed mainly in the nuclei,cytoplasm and membrane of the intestinal epithelial cells.Both the protein and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and omeprazole (P＜0.05),but the serum calcium and phosphate concentrations showed no obvious increase.Conclusion 1,25D3-MARRS binding protein,which is widely expressed with versatile functionalities,is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.关键词
1,25D3-MARRS结合蛋白/胃泌素/小肠钙磷吸收Key words
1,25D3-MARRS binding proteins/ gastrin/ calcium and phosphate uptake/ intestines引用本文复制引用
梁芬芬,刘翠平,黎丽旋,郭瑜,白岚..胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响[J].南方医科大学学报,2013,33(7):990-993,4.基金项目
国家自然科学基金(81170354) （81170354）
广东省科技计划项目(2011B031800195)Supported by National Natural Science Foundation of China (81170354). （2011B031800195）
