南方医科大学学报2013，Vol.33Issue(7)：994-998,5.DOI:10.3969/j.issn.1673-4254.2013.07.11
CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用
Effects of CXCR7-shRNA lentiviral vector on the growth and invasiveness of human hepatoma carcinoma cells in vitro
摘要
Abstract
Objective To explore the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation and invasion of human hepatoma carcinoma cells in vitro.Methods CXCR7-shRNA lentiviral vector was transfected into heptatocellular carcinoma HCCLM3 cells.The changes in mRNA and protein expression of CXCR7 in the transfected cells were investigated using real-time PCR and Western blotting,respectively,and MTT assay was employed to assess the cell proliferation changes.In vitro adhesion assay and transwell chamber test were used to observe the adhesion and invasiveness of HCCLM3 cells,respectively.Results Transfection of HCCLM3 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels (P＜0.01) and obvious suppression of the cell proliferative activity (P＜0.05).CXCR7-shRNA also significantly suppressed the invasiveness and adhesion of HCCLM3 cells (P＜0.01).Conclusion CXCR7 knock-down can significantly inhibit the proliferation and invasiveness of human hepatoma carcinoma cells in vitro,suggesting the value of CXCR7 as a potential target for hepatoma carcinoma therapy.关键词
肝癌/CXCR7-shRNA/生长/侵袭Key words
hepatoma carcinoma/ CXCR7-shRNA/ growth/ invasion引用本文复制引用
戴小珍,熊新,王兰,潘克俭,何浪,李红..CXCR7-shRNA慢病毒载体对人肝癌细胞生长及侵袭能力的抑制作用[J].南方医科大学学报,2013,33(7):994-998,5.基金项目
国家自然科学基金(81200917,81201702) （81200917,81201702）
四川省教育厅资助科研项目(10ZC092) （10ZC092）
重庆市自然科学基金项目(cstc2012jjA10139) （cstc2012jjA10139）
重庆大学"生物流变科学与技术"教育部重点实验室访问学者基金(CQKLBST-2012-003)Supported by National Natural Science Foundation of China (81200917,81201702). （CQKLBST-2012-003）
