南方医科大学学报2013，Vol.33Issue(7)：999-1003,5.DOI:10.3969/j.issn.1673-4254.2013.07.12
RNA结合蛋白La在宫颈癌组织中的表达及临床意义
La protein expression in cervical cancer tissues and its clinical significance
摘要
Abstract
Objetive To investigate the expression of La protein in cervical cancer tissues and explore its role in the occurrence and progression of cervical cancer.Methods The expression of La protein in cervical cancer and normal cervical tissues was detected by immunohistochemical staining.RNA interference technology was used to silence La protein expression in HeLa cells and the changes in cell proliferation,tumor sphere formation and cell cycles were investigated.Results The expression of La protein was significantly higher in cervical cancer tissues than in normal cervical tissues (61％ vs 9％,P＜0.05).Silencing La protein expression in HeLa cells caused significantly reduced the cell proliferation and lowered the tumor sphere formation rate from the control level of (17.1±1.92)％ to (6.3±0.45)％ (P＜0.05),resulting also in G0/G1 cell cycle arrest and reduced cyclin D1 protein expression.Conclusion The RNA binding protein La can promote the development of cervical cancer and may play a critical role in the carcinogenesis and progression of cervical cancer.关键词
宫颈癌/La蛋白/RNA干扰/细胞周期蛋白D1Key words
cervical cancer/ La protein/ RNA interference/ cyclin D1引用本文复制引用
陈昆仑,吴怡,李牧,李兰,高艳娥,高庆..RNA结合蛋白La在宫颈癌组织中的表达及临床意义[J].南方医科大学学报,2013,33(7):999-1003,5.基金项目
国家自然科学基金(81070536)Supported by National Natural Science Foundation of China (81070536). （81070536）
