南方医科大学学报2013，Vol.33Issue(8)：1132-1136,5.DOI:10.3969/j.issn.1673-4254.2013.08.07
β淀粉样蛋白通过升高ROS水平上调晚期糖基化终末产物受体的表达
β-amyloid protein up-regulates the expression of the receptor for advanced glycation end products by increasing ROS production
摘要
Abstract
Objective To investigate the mechanism of β-amyloid protein (Aβ) in regulating the expression of the receptor for advanced glycation end products (RAGE).Methods Aβ1～40 was injected into the bilateral hippocampus of rats,and 3 weeks later,the levels of reactive oxygen species (ROS) production were detected by flow cytometry.The expressions of RAGE,reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (gp91phox and p47phox),nuclear factor-κB (NF-κB),and inhibitor of κB (IκB) were measured by Western blotting.Results Injection of A1～40 caused a significant increase in the expressions of RAGE,gp91phox,p47phox,phospho-p47phox,phospho-IκBα,NF-κB and phospho-NF-κB in rat hippocampus but decreased the level of IκBα.Aβ1～40 injection also resulted in a significantly increased content of ROS in the hippocampus of the rats.Conclusion Aβ up-regulates the expression of RAGE in rat hippocampus via NADPH/ROS/NF-κB signaling pathway.关键词
阿尔茨海默病/晚期糖基化终末产物受体/β淀粉样蛋白/活性氧/还原型烟酰胺腺嘌呤二核苷酸氧化酶/核因子-κb/NF-κB抑制蛋白Key words
Alzheimer's disease/receptor for advanced glycation end products/β-amyloid protein/reactive oxygen species/reduced nicotinamide adenine dinucleotide phosphate oxidases/nuclear factor-κB/inhibitor of κB引用本文复制引用
孔卫娜,张佳,高维娟,刘清涛,周利明,柴锡庆..β淀粉样蛋白通过升高ROS水平上调晚期糖基化终末产物受体的表达[J].南方医科大学学报,2013,33(8):1132-1136,5.基金项目
国家自然科学基金(81273983) （81273983）
河北省自然科学基金(C2010001471) （C2010001471）
河北省高等学校科学技术研究青年基金(Q2012036)Supported by National Natural Science Foundation of China (81273983). （Q2012036）
