南方医科大学学报2013，Vol.33Issue(8)：1165-1168,4.DOI:10.3969/j.issn.1673-4254.2013.08.14
大鼠血浆中辣椒素的HPLC-MS/MS测定
Analysis of capsaicin in rat plasma using liquid chromatography-tandem mass spectrometry
摘要
Abstract
Objective To establish a simple high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS)system for determination of capsaicin in rat plasma.Methods Rat plasma (100 μl) was extracted with mixed solvent (ethyl acetate:acetone 85∶15) after addition of 50 ng/ml internal standard,and analyzed by Agilent 6460 Triple Quadrupole LC/MS system.The detection was performed with multiple reactions monitoring using electrospray ionization.The precursor/product ion transitions were monitored at m/z 306→137 (+Ion mode) for capsaicin.Verapamil was used as the internal standard at m/z455→165.Results The analysis time was only 1.5 min in the positive mode; the calibration curve was linear in the concentration range of 1.85-370 ng/ml.The lowest limit of quantification reached 1.85 ng/ml.The extraction recoveries were 77.34％,70.64％ and 78.02％ for the three quality control concentration levels (3.7,37,and 370 ng/ml),respectively.Conclusion The LC-MS/MS system is simple,accurate,reliable and time-saving for determination of trace amount of capsaicin in plasma.关键词
高效液相串联质谱/辣椒素/血浆/HPLC/MSKey words
high-performance liquid chromatography-tandem mass spectrometry/capsaicin/plasma/HPLC/MS引用本文复制引用
段炼,晏媛,刘媛,刘思佳,李国锋..大鼠血浆中辣椒素的HPLC-MS/MS测定[J].南方医科大学学报,2013,33(8):1165-1168,4.基金项目
国家自然科学基金(81173014) （81173014）
广东省科技计划项目(2010B030700014) （2010B030700014）
南方医院院长基金(2010B015)Supported by National Natural Science Foundation of China (81173014). （2010B015）
