南方医科大学学报2013，Vol.33Issue(8)：1232-1235,1239,5.DOI:10.3969/j.issn.1673-4254.2013.08.30
普伐他汀对P38MAPK信号通路介导的胰岛微血管内皮细胞炎症损伤的保护作用
Protective effects of pravastatin against P38MAPK signaling pathway-mediated inflammatory toxicity in islet microendothelial cells
摘要
Abstract
Objective To study the signaling pathways associated with lipopolysaccharide (LPS)-induced inflammation in islet microendothelial cells (IMECs) and the mechanism of pravastatin intervention.Methods IMECs exposed to LPS,SB203580,pravastatin,or SB203580 + pravastatin were examined for cell apoptosis with Hoechst staining and flow cytometry and for expression levels of total-p38,photophosphorylation-p38 (p-p38) and iNOS with Western blotting.Results The apoptosis rate and expression levels of total-p38,p-p38,iNOS in IMECs all increased after LPS exposure.Pravastatin,SB203580,and their combination significantly attenuated LPS-induced enhancement of cell apoptosis and total-p38,p-p38,and iNOS expressions in IMECs.Conclusion LPS-induced inflammatory toxicity in IMECs is associated with the activation of P38MAPK and iNOS/NO signaling pathways.Pravastatin can inhibit these pathways and suppress the apoptosis and necrosis of IMECs to relieve the cell inflammatory injuries.关键词
胰岛微血管内皮细胞/普伐他汀/炎症/P38MAPKKey words
islet microendothelial ceils/pravastatin/inflammation/P38MAPK引用本文复制引用
胡南,孙嘉,康远程,陈建生,罗丽珊,张菊嫦,陈松源,蔡德鸿..普伐他汀对P38MAPK信号通路介导的胰岛微血管内皮细胞炎症损伤的保护作用[J].南方医科大学学报,2013,33(8):1232-1235,1239,5.基金项目
广东省"大学生创新实验计划"项目(1212111004) （1212111004）
国家级大学生创新创业训练计划项目(201212121081) （201212121081）
广东省医学科研基金(2011B031800086) （2011B031800086）
广东省自然科学基金博士科研启动基金(S2012040006903) （S2012040006903）
广东省科技计划项目(C1030491) （C1030491）
