儿科药学杂志2013，Vol.19Issue(6)：47-50,4.
高效液相色谱法测定维地西林涂剂中盐酸丁卡因的含量
Determination of Hydrochloride Tetracaine in Verdicyline Paint by HPLC
摘要
Abstract
Objective:To establish a quality control method of hydrochloride tetracaine in verdicyline paint to ensure the safety of patients.Methods:By using HPLC method,C18 column (250 mm×4.6 mm,5 μm) was chosen; the mobile phase was methanol-0.4％ triethylamine solution (adjust pH to 3.0 with phosphoric acid) (55 ∶ 45) ; the flow rate was 1.0 mL/min; the detection wave length was 311 nm; the volume of sample was 20 μL; the column temperature was set at 30 ℃.Results:The curve of hydrochloride tetracaine was linear within a range of 0.494～ 12.350 μg/mL; the linear equation was Y=0.065X+0.0086 (r=0.9995) ; the average recovery was 99.8％ with RSD of 0.96％ (n=6).Conclusions:This method is fast,convenient,and with good reproducibility.It could be used to the content determination of hydrochloride tetracaine in verdicyline paint.关键词
维地西林涂剂/盐酸丁卡因/含量测定Key words
Verdicyline paint/ Hydrochloride tetracaine/ Content determination分类
医药卫生引用本文复制引用
李伏燕,彭芬芬,吴立兵..高效液相色谱法测定维地西林涂剂中盐酸丁卡因的含量[J].儿科药学杂志,2013,19(6):47-50,4.基金项目
湖北省重大科学技术鉴定成果(登记号:EK2012D 150018000359) （登记号:EK2012D 150018000359）
太和医院科研基金项目,编号:2009D34. （）
