福建林学院学报2013，Vol.33Issue(3)：220-224,5.
短葶山麦冬花蕾离体培养及快繁技术研究
Tissue culture and rapid propagation with pedicle of Liriope muscari (Decne.) Bailey
摘要
Abstract
Liriope muscari (Decne.) Bailey is a kind of ornamental plants and medicinal plants,and its wild resource has been damaged severely.So its seedlings have to be obtained by tissue culture to meet the market demand.In order to solve the problem of disinfection,a tissue culture system of L.muscari with its pedicles was built by screening different disinfection methods,different culture medium formulas and different matrixes for transplanting.Experimental results showed as follows.The most suitable procedure for sterilization was to soak the plants into 0.1％ HgCl2 solution for 10 min and then wash them with sterile water for 5 times after soaking them into 75％ alcohol disinfection for 30 s and washing with sterile water for 3 times.The optimum initial medium was MS +1 mg·L-16-BA + 0.2 mg·L-1NAA,the optimum subculture medium was MS +4 mg·L-16-BA + 0.2 mg·L-1NAA,and the optimum rooting medium was 1/2 MS + 1 mg·L-1IBA +30 g·L-1 sucrose.The survival rate up to 93.33％ when the ratio of red loam,perlite,sand mixing was 6:3:1.关键词
短葶山麦冬/组织培养/快繁增殖Key words
Liriope muscari (Decne.)Bailey/ tissue culture/ rapid propagation proliferation分类
农业科技引用本文复制引用
陈凌艳,董雯,荣俊冬,郑郁善..短葶山麦冬花蕾离体培养及快繁技术研究[J].福建林学院学报,2013,33(3):220-224,5.基金项目
福建省重大专项基金资助项目(2007SZ0001). （2007SZ0001）
