福建农业学报2013，Vol.28Issue(6)：597-603,7.
PCR-DGGE技术在智能化沼气池微生物多样性研究中的应用
Application of PCR-DGGE on Analyzing Microbial Diversity in Intelligent Biogas Digester
摘要
Abstract
The bacterial community and diversity were revealed by using PCR-DGGE technique,which were associated with biogas slurry of up-flow intelligent biogas digester.The results showed that the majority genus of bacteria were Empedobacter sp.,Thermomonas sp.,Duganella sp.,Gordonia sp.,Bacillus sp.,Bacteroidetes bacterium and anaerobic bacterium.The sequences of 16S rDNA predominant bands in DGGE were compared with NCBI database.The results showed that the bacterial community was abundant in up-flow intelligent biogas digester,but its community structure was changed variously with fermentation time phases and fermentation layer phases in intelligent biogas digester.The most of predominant bacterial genus were non-cultural bacteria.The results suggest that the DGGE technique is reliable in applying to analyze microbial diversity in intelligent biogas digester.关键词
PCR-DGGE/智能化/沼气池/微生物群落Key words
PCR-DGGE/ pig farm/ intelligent/ biogas digester/ bacterial communities分类
农业科技引用本文复制引用
徐庆贤,官雪芳,林碧芬,林斌,钱蕾..PCR-DGGE技术在智能化沼气池微生物多样性研究中的应用[J].福建农业学报,2013,28(6):597-603,7.基金项目
国家科技部科技人员服务企业项目(2009GJC40010) （2009GJC40010）
福建省发改委农业"五新"推广项目(2010452) （2010452）
