分析测试学报2013，Vol.32Issue(7)：810-816,7.DOI:10.3969/j.issn.1004-4957.2013.07.005
荧光摩尔吸收系数法测定洗护用品中烷基酚聚氧乙烯醚
Determination of Alkyphenol Ethoxylates in Detergent Products and Cosmetics with Quantitative Model Based on Fluorescence Molar Absorption Coefficient
摘要
Abstract
A hybrid high performance liquid chromatographic method was developed for the determination of alkyphenol ethoxylates(NPnEO and OPnEO,n =0-30)in detergent products and cosmetics.Different types of samples were extracted by ultrasonic-assisted extraction using a mixture of acetonitrile-water(8 ∶ 2).The chromatographic separation was conducted with a reverse phase column of C18(Poroshell 120 EC-C18,75 mm ×4.6 mm,2.7 μm) coupled with a HILIC-Silia column (Atlantis HILIC Slica,250 mm ×4.6 mm,5 μm) by gradient elution using acetonitrile-10 mmoL/L ammonium acetate as mobile phase.The detection was performed with a fluorescence detector (FLD).A quantitative model was established based on fluorescence molar absorption coefficients of NPnEO and OPnEO.With the quantification of the hybrid standard solution of NPnEO and OPnEO,the fluorescence molar absorption coefficients of NPnEO and OPnEO were determined.The average molecular weight of NPnEO and OPnEO in real samples were measured,and the concentrations of the analytes were calculated with the fluorescence molar absorption coefficients.With this method,the average recoveries for APEOs ranged from 99.0％ to 105.7％ with relative standard deviations no more than 12.5％,and the limit of quantitation were both 40.0 mg/kg.关键词
洗涤用品/化妆品护理用品/烷基酚聚氧乙烯醚/荧光摩尔吸收系数/高效液相色谱Key words
detergent products/cosmetics/alkyphenol ethoxylates (APEOs)/fluorescence molar absorption coefficient/high performance liquid chromatography分类
化学化工引用本文复制引用
席绍峰,李慧勇,谭建华,王继才,熊小婷,赵田甜,郭长虹,解启来..荧光摩尔吸收系数法测定洗护用品中烷基酚聚氧乙烯醚[J].分析测试学报,2013,32(7):810-816,7.基金项目
质检公益性行业科研专项项目(201010048-03) （201010048-03）
