分析测试学报2013，Vol.32Issue(7)：872-876,5.DOI:10.3969/j.issn.1004-4957.2013.07.016
基于谷胱甘肽-壳聚糖自组装的葡萄糖生物传感器
Glucose Biosensor Based on the Chitosan/Glutathione Composite Film
摘要
Abstract
A chitosan/glutathione composite film combined with glucose oxidase was employed to fabricate an amperometric glucose biosensor.The enzyme electrode was characterized by cyclic voltammetry.The experimental results showed that,chitosan and glutathione composite film can assist in electron transfer,improve response electrode current.Using orthogonal table L9 (34) experiment design,the best experimental conditions were obtained by visual analysis.Under the optimized conditions,the sensor has a good linear relationship with concentration of glucose solution in the range of 1-18 mmol/L,the detection limit is 1.3 mmol/L.Experiments showed that,this sensor has the advantages of fast response,good stability and selectivity,and is suitable for the determination of glucose in urine sample.关键词
金电极/壳聚糖/葡萄糖氧化酶/生物传感器/谷胱甘肽/正交试验Key words
gold electrode/chitosan/GOx/biosensor/glutathione/orthogonal array分类
化学化工引用本文复制引用
高先娟,王怀生,蔡青云..基于谷胱甘肽-壳聚糖自组装的葡萄糖生物传感器[J].分析测试学报,2013,32(7):872-876,5.基金项目
山东省教育厅高校科研发展计划项目(J13LD59) （J13LD59）
