分析测试学报2013，Vol.32Issue(7)：886-889,4.DOI:10.3969/j.issn.1004-4957.2013.07.019
液相色谱-串联质谱法同时测定人血清中皮质酮与皮质醇
Determination of Corticosterone and Hydrocortisone in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry
马晓斐 1吕品 2李挥 1张岩1
作者信息
- 1. 河北省食品质量监督检验研究院,河北石家庄050091
- 2. 河北医科大学法医学系,河北石家庄050017
- 折叠
摘要
Abstract
A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of corticosterone and hydrocortisone in serum was developed.The fat in serum was removed with hexane and extracted with methyl ether.Corticosterone and hydrocortisone were separated on an Agilent Eclipse Plus-C18 column(150 mm ×2.1 mm,3.5 μm) and detected by chromatography coupled with tandem mass spectrometry in positive ion mode with mobile phase of acetonitrile-0.1％ formic acid(containing 0.01 mol/L ammonium formate).The flow rate of mobile phase was set at 0.3 mL/min.The calibration curves of two target compounds were in the range of 0.5-200 ng/mL,with correlation coefficients(r2) of 0.999 7 and 0.999 4,respectively.The recoveries for the spiked sample at the levels of 5.0,25.0 and 75.0 ng/mL for corticosterone and 0.5,2.5,10.0 ng/mL for hydrocortisone,ranged from 86.6 ％ to 102.7％ with relative standard deviations not more than 7.1％.The limits of detection (S/N=3) for corticosterone and hydrocortisone were 0.1 ng/mL and 0.05 ng/mL,respectively.This method was simple,sensitive and accurate,and was suitable for the determination of corticosterone and hydrocortisone in serum sample.关键词
血清/液相色谱串联质谱/皮质酮/皮质醇Key words
serum/liquid chromatography tandem mass spectrometry/corticosterone/hydrocortisone分类
化学化工引用本文复制引用
马晓斐,吕品,李挥,张岩..液相色谱-串联质谱法同时测定人血清中皮质酮与皮质醇[J].分析测试学报,2013,32(7):886-889,4.
