分析测试学报2013，Vol.32Issue(7)：894-897,4.DOI:10.3969/j.issn.1004-4957.2013.07.021
硫辛酸片含量与降解物的高效液相色谱测定研究
Determination of Thioctic Acid Tablets and Their Degradation Products by HPLC
摘要
Abstract
A high-performance liquid chromatographic (HPLC) method for the determination of relative substances in thioctic acid tablets was developed.The separation of analyte was performed on a Waters XBridge column(5 μm,150 mm × 4.6 mm) with phosphate buffer(pH value was adjusted to 2.5 with phosphoric acid)-acetonitrile-methanol(50 ∶ 45 ∶ 5) as mobile phase,at a flow rate of 1.0 mL/min and an injection volume of 20 μL.The photo-diode array detector(PDA) was set at 220 nm,with full scan range of 200-400 nm.The column temperature was ambient.Under the above chromatographic conditions,the calibration curve was linear(r =0.999 9) in thioctic acid concentration range of 0.4-20.0 mg/L.The limit of detection (LOD) for thioctic acid was 0.2 mg/L and the limit of quantitation(LOQ) was 0.6 mg/L.The relative standard deviation(RSD,n =12)was 3.4％,and the average spiked recovery was 100.9％ with RSD of 2.0％.The proposed method could be applied in the determination of relative substances in thioctic acid tablets with the advantages of simplicity,rapidity,accuracy,good selectivity and high sensitivity.关键词
硫辛酸片/硫辛酸/降解物/高效液相色谱法Key words
thioctic acid tablet/thioctic acid/degradation products/HPLC分类
化学化工引用本文复制引用
叶汝汉,张怡,彭蕾,孙晋红,张嘉颖,张慧影,伍迪科,张萍..硫辛酸片含量与降解物的高效液相色谱测定研究[J].分析测试学报,2013,32(7):894-897,4.基金项目
广东省科技计划项目(2010A040209001) （2010A040209001）
