广东医学2013，Vol.34Issue(14)：2125-2128,4.
高锶矿泉水的细胞毒性研究
Cytotoxicity study of strontium mineral water
摘要
Abstract
Objective To investigate the effects of sttino of strontium mineral water (SMW).Methods LO2,HK-2 and HUVEC cells were cultured in vitro with normal Dulbecco's Modified Eagle's Medium (DMEM) for 18 hours,and subsequently with conditioned medium containing SMW (2,4,6 and 8 mg/L) or double distilled water (DDW).The cell proliferation was observed by cell counting kit-8 (CCK-8) assay on the 1st,2nd,3rd,4th,5th and 7th days.The cytotoxicity was evaluated by cell relative growth rate according to the United States pharmacopoeia.Extracellular vein endothelial growth factors (VEGF) were assessed by enzyme-linked immunosorbent assay.Results The cytotoxicity grades of SMW of 2,4 and 6 mg/L were 0 ～ 1 on LO2,HK-2 or HUVEC cells,while they were 2 ～ 3 when SMW of 8 mg/L on cell at early or later period of proliferation.The proliferation peaks of LO2 cells came significantly earlier in SMW of 4 mg/L and 6 mg/L than in those in control group,and kept vigorous proliferation significantly longer (P ＜ 0.05).Significantly prolonged growth cycle was observed in HK-2 cells with SMW of 4,6,or 8 mg/L (P ＜ 0.05),which was significantly most prominent with SMW of 4 mg/L (P ＜ 0.05).The proliferation activity of HUVEC cells in SMW of 2 and 4 mg/L was significantly increased (P ＜0.05) on the 3rd day; however,it was significantly inhibited with SMW of 8 mg/L(P ＜ 0.05).The total VEGF in cells in SMW groups were significantly lower than control group (P ＜ 0.05),though there was no significant difference among SMW groups (P ＞ 0.05).Conclusion SMW has little cytotoxicity on LO2,HK-2 and HUVEC cells at the concentration lower 8 mg/L,even with beneficial effects on cell proliferation at certain concentration,which is prominent at 4 mg/L.VEGF may not be the major induced-proliferative factor on HUVEC.关键词
锶矿泉水/细胞毒性/肝细胞/肾小管细胞/血管内皮细胞/增殖/血管内皮生长因子Key words
strontium mineral water/ cytotoxicity/ hepatocytes/ human renal tubular epithelial cell/ human umbilical vein endothelial cells/ proliferation/ vascular endothelial growth factor引用本文复制引用
蔺艳,张莹茜,盘强文,冯志强,李著华,赵成涛,冉兵..高锶矿泉水的细胞毒性研究[J].广东医学,2013,34(14):2125-2128,4.基金项目
四川省卫生厅科研课题(编号:090188) （编号:090188）
