广东药学院学报2013，Vol.29Issue(3)：273-276,4.DOI:10.3969/j.issn.1006-8783.2013.03.013
茶枝柑皮提取物中黄酮类成分的含量测定
Determination of flavonoids from Citrus Reticulata ‘Chachi’ peel extract
摘要
Abstract
Objective To establish a HPLC method to determine flavonoids from Citrus reticulata 'Chachi' peel extract.Methods Hesperidin,nobiletin and tangeretin were determined on Phenomenex Luna C18 (250 mm × 4.6 mm,5 μm) column and detected at 283 nm and 330 nm.The mobile phase was consisted of acetonitrile and 0.2％ acetic acid.The column temperature was 25 ℃.Results The linear range of hesperidin was within 8.240-164.8 μg/mL (r =0.999 7),and the recovery was 97.3％ with RSD =2.6％.That of nobiletin was within 8.030-160.6 μg/mL (r =0.999 8),and the recovery was 99.1％ with RSD =0.9％.That of tangeretin was within 8.210-164.2 μg/mL (r =0.999 8),and the recovery was 97.6％ with RSD =1.3％.Conclusion This method is simple and accurate,which may provide a reference for the quality control of Citrus reticulate 'Chachi' peel extract.关键词
茶枝柑皮提取物/橙皮苷/川陈皮素/橘皮素/含量测定Key words
Citrus Reticulata ‘Chachi’ peel extract/ hesperidin/ nobiletin/ tangeretin/ quantitative determination分类
医药卫生引用本文复制引用
甘伟发,黄庆华,吉国辉,定力..茶枝柑皮提取物中黄酮类成分的含量测定[J].广东药学院学报,2013,29(3):273-276,4.基金项目
珠海市科技工贸和信息化局企业技术创新项目[珠经贸字(2009)414号] （2009）
