广东药学院学报2013，Vol.29Issue(3)：310-313,4.DOI:10.3969/j.issn.1006-8783.2013.03.023
外源基因转染血管内皮细胞条件优化
Optimization of exogenous gene transfection in human umbilical vein endothelial cells
摘要
Abstract
Objective To compare the efficiency of cationic liposome-and electroporation-mediated exogenous gene transfection in human umbilical vein endothelial cells (HUVEC),and optimize the transfection condition.Methods HUVEC cells were transfected with plasmid DNA,carried green fluorescent protein (GFP) gene,by LipofectaminTM 2000 or electroporation.The GFP expression and transfection efficiency were examined by fluorescent microscope at 48 h after transfection.Results The hypofluorescence signal was observed in the LipofectamineTM 2000-treated group,however the hyperfluorescence signal was detected in the electroporation group.The optimal condition in the electroporation group included the electric field strength 1 100 V,pulse time 20 ms and electric shock 2 times.Conclusion The efficiency of exogenous gene transfection with electroporation is higher than cationic liposome,which is suitable for exogenous gene transfection in HUVEC cells.关键词
人脐静脉血管内皮细胞/基因转染/阳离子脂质体/电穿孔Key words
HUVEC / gene transfection / cationic liposome / electroporation分类
生物学引用本文复制引用
章倩倩,丁一,刘红英,刘翼龙,陈胜霞,胡曦文,王丽京..外源基因转染血管内皮细胞条件优化[J].广东药学院学报,2013,29(3):310-313,4.基金项目
国家自然科学基金(31200896) （31200896）
广东省医学科研基金(B2012181) （B2012181）
广东省自然科学基金(S2012040007658) （S2012040007658）
