广东药学院学报2013，Vol.29Issue(3)：314-317,4.DOI:10.3969/j.issn.1006-8783.2013.03.024
联合靶向Survivin和Livin的双重siRNA表达载体的构建及其活性鉴定
Construction and activity of the dual-siRNA-expressed vector targeting Survivin and Livin
摘要
Abstract
Objective To construct a dual-siRNA expression vector targeting Survivin and Livin and identify its activity,and provide evidence for down-regulation of both genes simultaneously.Methods The published siRNA sequences targeting Survivin and Livin,respectively,were chosen to construct the dual-siRNA-expressed vector pSilencer2.1-Sur-Liv.This vector was then transfected into HepG2 cells and the mRNA levels of these two genes were detected.This vector was also transfected into Hela reporter cells,which expressed DsRed2-SURVIVIN and GFP-LIVIN,for examining the florescence.Results The recombinant vector pSilencer2.1-Sur-Liv was successfully constructed.After transfected with pSilencer2.1-Sur-Liv,the mRNA levels of Survivin and Livin in HepG2 cells were obviously decreased and the florescence in Hela reporter cells was reduced.Conclusion The dual-siRNA-expressed vector targeting Survivin and Livin has been constructed successfully and may effectively suppress the expression of Survivin and Livin.关键词
RNA干扰/Survivin/Livin/双重siRNA/基因治疗Key words
RNA interference/ Survivin/ Livin/ dual-siRNA / gene therapy分类
生物科学引用本文复制引用
陈孟璋,郭芬..联合靶向Survivin和Livin的双重siRNA表达载体的构建及其活性鉴定[J].广东药学院学报,2013,29(3):314-317,4.基金项目
广东省优秀博士论文作者资助项目(sybzzxm201051) （sybzzxm201051）
广东药学院科研启动基金(2007SMK02) （2007SMK02）
