南方农业学报2013，Vol.44Issue(6)：893-897,5.DOI:10.3969/j:issn.2095-1191.2013.6.893
翅碱蓬脱水素基因克隆及表达载体构建
Cloning and expression vector construction of Suaeda salsa dehydrin gene
摘要
Abstract
[Objective]Cloning and expression vector of Suaeda salsa dehydrin gene were conducted to provide references for enduring-salt research of Suaeda salsa.[Method]Total RNA of Suaeda salsa was extracted,and the gene of Suaeda salsa dehydration was obtained using homology cloning and RACE separation methods.The plant expression vector pBI121-DHN of this gene was constructed and transformed into Agrobacterium tumefaciens for genetic transformation.[Result]The full-length cDNA of Suaeda salsa dehydrin gene was 847 bp (GenBank sequence number.KC013239) and named SsDHN.5'-UTR in the full length of SsDHNwas 61 bp,and 3'-UTR zone was 108 bp including a polyA tail with 29 bp.Its ATG,the initiation codon,was located in 62 bp,and TAA,termination codon,was in 737 bp and encoded 225 amino acids.The results of deduced amino aicd comparison indicated that gene sequence of SsDHN had 35％-48％ similarity with the other known plant DHN.The cluster analysis results showed that phylogenetic relationship between SsDHN and DHN of Arabidopsis thaliana and Capsella bursapastoris was genetically close.However,SsDHN was not closely related to Coffea canephora and Rhododendron catawbiense.[Conclusion]The dehydrin gene of Suaeda salsa was cloned successfully.The plant expression vector was successfully constructed and can be used for the plant genetic improvement in salt resistance of Suaeda salsa.关键词
盐地碱蓬/脱水素蛋白/基因克隆/表达载体构建Key words
Suaeda salsa/dehydration grain protein/gene cloning/expression vector construction分类
农业科技引用本文复制引用
马慧,李丽丽,祝红艳,钟鸣,陈丽静,郭志富..翅碱蓬脱水素基因克隆及表达载体构建[J].南方农业学报,2013,44(6):893-897,5.基金项目
辽宁省农业生物技术重点实验室基金项目(200556) （200556）
辽宁省农业科学院博士后基金项目(2012) （2012）
