南方农业学报2013，Vol.44Issue(6)：903-908,6.DOI:10.3969/j:issn.2095-1191.2013.6.903
赤霉素、光照及基因型对花生体细胞胚诱导和植株再生的影响
Effect of gibberellin, light and genotype on peanut somatic embryogenesis and plantlet regeneration
摘要
Abstract
[Objective]Peanut in vitro regeneration system was optimized to provide technical support for its genetic transformation and mutagenesis.[Method]The effect of gibberellin,light and genotype on somatic embryogenesis and plantlet regeneration was studied using Guihua peanut varieties as materials,leaflets as explants,MS+10 mg/L 2,4-D as induction medium,and MS+3 mg/L 6-BA+0.8 mg/L NAA+(0-15 mg/L) GA3 as germination medium.[Result]The frequency of embryogenesis was significantly higher (light:dark=14 h:10 h) comparing with complete dark,and there was an increase range of percent point from 7.5 to 37.5 for different peanut varieties,respectively.The highest frequency of embryogenesis was 62.8％ for Guihua 26,and the lowest was 21.7％ for Guihua 833.The frequency of shoot development increased with a concentration range of GA3 from 5 mg/L to 15 mg/L.The 5 mg/L of GA3 was the best for Guihua 26 and Guihua 771 with a frequency of shoot development of 42.8％ and 35.3％,respectively,and the 15 mg/L of GA3 was the best for Guihua 836 and Guihua 1026 and Guihua 833.Their frequency of shoot development was 38.7％,33.3％ and 26.4％,respectively.Among all the combinations of variety and GA3 concentration,the highest frequency of shoot development was Guihua 26 and 5 mg/L of GA3,and the next was Guihua 836 and 15 mg/L of GA3.[Conclusion]Appropriate light propels the development of peanut in vitro embryo.GA3 can increase the frequency of shoot development from somatic embryos,and Guihua 26 and Guihua 836 are the best peanut varieties among the tested genotypes for somatic embryogenesis and plant regeneration.关键词
花生/体细胞胚诱导/植株再生/赤霉素/光照/基因型Key words
peanut/somatic embryogenesis induction/plantlet regeneration/gibberellin/light/genotype分类
农业科技引用本文复制引用
蒋菁,熊发前,唐秀梅,钟瑞春,贺梁琼,李忠,韩柱强,唐荣华..赤霉素、光照及基因型对花生体细胞胚诱导和植株再生的影响[J].南方农业学报,2013,44(6):903-908,6.基金项目
广西农业科学院基本科研业务专项项目(桂农科2012YM22) （桂农科2012YM22）
广西农业科学院公益性维持费项目(桂农科2013GW02) （桂农科2013GW02）
