南方农业学报2013，Vol.44Issue(6)：909-913,5.DOI:10.3969/j:issn.2095-1191.2013.6.909
越南凉粉草无菌芽增殖生长的影响因子
Study on effect factors for multiplication and growth of aseptic buds of Vietnam Mesona blumes
摘要
Abstract
[Objective]The effect factors on multiplication and growth of the aseptic buds were studied in order to provide technical references for rapid propagation system in vitro of Vietnam Mesona blumes.[Method]The experiments were carried out from two aspects of media directions (different basic media,sucrose concentration and cytokinins) and explant materials (different inoculation positions).[Result]Comparison of the basic media of MS,Miller and modified White displayed that MS medium showed the best effect on bud multiplication and growth of the aseptic buds,in which bud proliferation multiple was 8.53 times.While sucrose concentration was 20.0 g/L,bud proliferation multiple peaked at 8.34 times.When sucrose concentration reached 25.0 g/L,bud proliferation multiple showed 7.05 times and bud growth was the best.The bud multiplication effect of CPPU was better than that of 6-BA and KT,in which bud proliferation multiple reached 9.80 times.Comparing the inoculated materials from stem upper position,the middle part of the stem and the stem base position,the bud multiplication effect of stem upper position was the best,in which the bud proliferation multiple was 9.35 times and the bud growth exhibited the best status.[Conclusion]It was suitable to the bud multiplication and growth of Vietnam Mesona blumes that the basic medium was MS medium,20.0-25.0 g/L sucrose concentration and 0.5 mg/L CPPU.The proper inoculated material was the stem upper position.关键词
越南凉粉草/无菌苗/芽增殖/影响因子Key words
Mesona blumes/aseptic seedling/bud multiplication/effect factor分类
农业科技引用本文复制引用
韦鹏霄,谭木秀,岑秀芬,覃柳兰,刘洁云,周芳芳..越南凉粉草无菌芽增殖生长的影响因子[J].南方农业学报,2013,44(6):909-913,5.基金项目
广西梧州市科学研究与技术开发计划项目(200901024) （200901024）
