南方农业学报2013，Vol.44Issue(6)：989-993,5.DOI:10.3969/j:issn.2095-1191.2013.6.989
广西优良珍贵树种灰木莲的组织培养
Tissue culture of rare species Manglietia glauca in Guangxi
摘要
Abstract
[Objective]Rapid regeneration system for Manglietia glauca was explored to provide references for its further development and utilization,seedling replacement and tissue culture of other magnoliaceae plants.[Method]The tissue culture of Manglietia glauca was studied using stems with buds as explants.The explants were cultivated in minimal medium with different types and concentrations of plant growth regulators.[Result]The axillary bud of Manglietia glauca explants could be induced in 1-5 medium.The best shoot induction medium was 3 (MS+6-BA 0.5 mg/L+NAA 0.1 mg/L) with the highest induction rate of 89.3％.The multiplication coefficient of plantlets in 10-13 medium added with kinetin (KT) were 2.2-2.7,while the coefficients in 6-9 medium without KT were 1.8-2.2.With the highest multiplication coefficient of 2.7,the optimum medium for multiplication was 11 (MS+6-BA 0.3 mg/L+NAA 0.05 mg/L+KT 1.0 mg/L).The roots of plantlets could be induced on medium 17-21 with the concentration of NAA higher than 0.5 mg/L The rooting rate were 33.3％,45.4％ and 58.3％,while NAA in the medium were 0.5,1.5 and 2.0 mg/L,within which the best rooting medium was 21 (1/2MS+NAA 2.0 mg/L).[Conclusion] The regeneration system of Manglietia glauca was basically established.The plantlets grew well because of high induction rate and high propagation coefficient.The rooting induction should be further studied.关键词
珍贵树种/灰木莲/组织培养/增殖系数/生根率Key words
rare species/Manglietia glauca/tissue culture/multiplication coefficient/rooting rate分类
农业科技引用本文复制引用
乔梦吉..广西优良珍贵树种灰木莲的组织培养[J].南方农业学报,2013,44(6):989-993,5.基金项目
广西大学科研基金项目(XJZ110610) （XJZ110610）
