南方农业学报2013，Vol.44Issue(6)：1026-1029,4.DOI:10.3969/j:issn.2095-1191.2013.6.1026
温度对家蚕微孢子虫(Nosema bombycis)孢子体外发芽的影响
Influence of temperature on in vitro germination of Nosema bombycis spore
摘要
Abstract
[Objective]The present study was conducted to investigate the effect of temperature on the germination of Nosema bombycis spore in vitro so as to provide information for preventing and treating pebrine and relevant microsporidia fundamental research.[Method]The spores were stimulated to germinate with the way of K2CO3-KHCO3 at different temperature (5℃,15℃,25℃,35℃) at different period,then germination rate from different treatment was observed.[Result]In earlier stage,the germination rate of Nosema bombycis spores was 3.88％,15.66％,31.91％ and 58.75％,respectively.The germination rate of Nosema bombycis spores went up with the rising temperature.The germination rate of Nosema bombycis spores was 53.33％,38.17％,25.07％ and 6.08％ in stimulated stage,respectively,and it went down with the rising temperature.The germination rate of Nosema bombycis spores was 44.63％,41.29％,43.53％ and 48.82％ in rearing stage,respectively,and there was no significant difference.[Conclusion]Temperature had certain effect on germination of Nosema bombycis spores in vitro.Throughout the entire process of spore in vitro germination,low temperature in earlier stage and high temperature in stimulated stage could inhibit the germination of Nosema bombycis spores,and high temperature in earlier stage and low temperature in stimulated stage could promote its germination.关键词
家蚕/微孢子虫/温度/孢子体外发芽/K2CO3-KHCO3法Key words
Bombyx mori/microsporidia/temperature/spore in vitro germination/K2CO3-KHCO3 method分类
农业科技引用本文复制引用
黄旭华,朱方容,潘志新,汤庆坤,罗梅兰,罗坚,陈小青,黄深惠..温度对家蚕微孢子虫(Nosema bombycis)孢子体外发芽的影响[J].南方农业学报,2013,44(6):1026-1029,4.基金项目
国家星火计划项目(2011GA7900001) （2011GA7900001）
广西自然科学基金项目(桂科自0832179,桂科自1013072) （桂科自0832179,桂科自1013072）
