中医学报2013，Vol.28Issue(8)：1161-1164,后插1,5.
柴胡疏肝散合参苓白术散对非酒精性脂肪性肝病大鼠肝组织LXRα mRNA及蛋白表达的影响
Influence of Soothing Liver and Invigorating Spleen Recipes on LXR Alpha mRNA of Liver Tissue and Protein Expression of NAFLD Rats
摘要
Abstract
Objective:To study the influence of soothing liver and invigorating spleen recipes on LXR alpha mRNA of liver tissue and protein expression of NAFLD rats.Methods:55 SD rats were randomly divided into the five groups,namely,normal group of 10 rats,model group of 15 rats,soothing liver group of 10 rats,invigorating spleen group of 10 rats,comprehensive group of 10 rats.The normal group was given basal feed,and other groups were given intragastric administrated with high fat emulsion 10 mL · kg-1 one time at 8:00 every morning.All rats took feed and drunk water at freedom for continuous for 8 weeks.Rats of drug intervention groups were given the corresponding drugs of 10 mL per kilogram weight,and the dosage of administration was discounted according to the human and animal body surface area.The soothing liver group was intragastric administrated with Caihu Shugan San (containing crude drug dose of 0.32 kg · L-1),and the invigorating spleen group was intragastric administrated with Shenling Baizhu Power (containing crude drug dose of 1.00 kg · L-1).the comprehensive group was intragastric administrated with Caihu Shugan San and Shenling Baizhu Power (Containing crude drug dose of 1.19 kg · L-1).The normal group and the model group were given intragastric administrated with the same dose of distilled water,1 time every afternoon.The animals were sacrificed at 8weeks after medication.abdominal aortic blood,and blood lipid and liver function were tested by fully automatic biochemical analyzer.Conventional HE staining was used to observe the pathological changes of hepatic tissue ;RT-PCR method was used for detection the expression of liver tissue LXR alpha mRNA ;Immunohistochemical method was applied to detect the expression level of liver tissue LXR alpha protein.Results:Compared with the normal group,hepatocyte steatosis of the model group rats was apparent,and blood lipid and liver function of the model group had different degrees of increase (P ＜ 0.05,P ＜ 0.01),and rat liver tissue LXR alpha mRNA and protein expression level of the model group increased significantly(P ＜0.01) ;Blood lipid,liver function and liver tissue LXR alpha mRNA and protein expression levels in each medication administration group were significantly lower than that in the model group(P ＜0.05 or P ＜0.01),and among which,the invigorating spleen group was the most obviously declined.Conolusion:Soothing liver and invigorating spleen recipes has good therapeutical effect,and its mechanism may be related to its downgrade the expression level of liver LXR alpha.关键词
非酒精性脂肪性肝病/柴胡疏肝散/参苓白术散/肝X受体α基因/大鼠Key words
non-alcoholic fatty liver disease/ caihu shugan san/ shenling baizhu power /LXRα mRNA/ rat分类
医药卫生引用本文复制引用
弯玲,王嘉锋,杨钦河,王文晶,冯高飞,何秀敏,闫海震,黄进..柴胡疏肝散合参苓白术散对非酒精性脂肪性肝病大鼠肝组织LXRα mRNA及蛋白表达的影响[J].中医学报,2013,28(8):1161-1164,后插1,5.基金项目
广州市中医药中西医结合科研课题(编号:2010A44) （编号:2010A44）
