中医学报2013，Vol.28Issue(8)：1178-1179,2.
白芷中欧前胡素和异欧前胡素的含量测定
Determination of Imperatorin and Isoimperatorin in Radix Angelicae Dahuricae
摘要
Abstract
Objective:To establish a method for the simultaneous determination of imperatorin and isoimperatorin in radix angelicae dahuricae by HPLC.Methods:The separation was performed on a Venusil XBP C18 (4.6 mm x 250 mm,5 μm),column using methanol-wa-ter(60∶ 40)as mobile phase at 30 ℃,and the flow rate was 1.0 mL · min-1.The detection wavelengths was set at 304 nm.Results:The linear relation was in the rang of 0.15 ～ 1.80 μg(r =0.999 5)for imperatorin,0.10 ～ 1.20 μg(r =0.999 6)for isoimperatorin,respectively.Their average recoveries were 100.2％ (RSD was 1.57％),99.4％ (RSD was 1.21％).Conclusion:The method is simple,reliable,and reproducible for the content determination of imperatorin and isoimperatorin in radix angelicae dahuricae.关键词
白芷/欧前胡素/异欧前胡素/含量测定/高效液相色谱法Key words
radix angelicae dahuricae/ imperatorin / isoimperatorin/ content determination/ HPLC分类
医药卫生引用本文复制引用
万焱,马国柱,冯素香..白芷中欧前胡素和异欧前胡素的含量测定[J].中医学报,2013,28(8):1178-1179,2.基金项目
国家自然科学基金重大项目(编号:69317668) （编号:69317668）
