河南农业科学2013，Vol.42Issue(5)：158-162,5.
T-2毒素人工抗原的合成及鼠源多克隆抗血清的制备
Preparation of T-2 Toxin Artificial Antigen and Mouse Polyclonal Antiserum
摘要
Abstract
To prepare high sensitive and specific polyclonal antiserum against T-2 toxin,T-2 toxin artificial antigens were synthesized and identified by animal immunization in this study.T-2 toxin was converted to T-2-hemisuccinate (T-2-HS) and coupled with carrier pro tein BSA and OVA using EDC method,and then the immune antigen T-2-BSA and detection antigen T-2-OVA were obtained,respectively.To prepare the poly-antiserum against T-2,BALB/c mouse was immunized with T-2-BSA at the doses of 30 μg and 50μg for four times at the intervals of four weeks.Ten days after the last immunization,the titer of antiserum was determined by indirect ELISA.The results showed that antiserum of high titers above 1 ∶ 104 were produced in six mice.The antiserum of mouse # 2 had the best sensibility.Its IC50 was 324 ng/mL and it had good specificity.In this study,T-2 toxin artificial antigens were successful synthesized and the poly-antiserum with high sensibility and specificity was obtained,providing a foundation for the further study.关键词
T-2毒素/人工抗原/多抗血清/ELISAKey words
T-2 toxin/ artificial antigen/ polyclonal antiserum/ ELISA分类
农业科技引用本文复制引用
王坤,李清州,胡骁飞,李文君,孙亚宁,王方雨,职爱民,邓瑞广,张改平..T-2毒素人工抗原的合成及鼠源多克隆抗血清的制备[J].河南农业科学,2013,42(5):158-162,5.基金项目
国家科技支撑计划项目(2011BAK10B01) （2011BAK10B01）
农业部公益性行业(农业)科研专项(201203040) （农业）
农业部公益性行业(农业)科研专项(201003008) （农业）
