河南农业科学2013，Vol.42Issue(7)：68-71,83,5.
解淀粉芽孢杆菌X-278菌株抗菌蛋白初步分析
Primary Analysis of Antifungal Proteins Produced by Bacillus amylolique faciens X-278 against Verticillium dahliae
摘要
Abstract
In order to study the characteristics of antifungal proteins isolated from Bacillus amyloliquefaciens X-278,crude proteins were extracted via precipitation of the cell culture of strain X-278 with ammonium sulphate,and the protease stability and thermal stability were determined.The result showed that the crude proteins of strain X-278 had strongest control effect on cotton Verticillium wilt using ammonium sulphate at 50％ saturation,with the inhibition zone diameter of 18.9 mm.The crude protein extracts were relatively insensitive to proteinase K,but more sensitive to trypsin.The activity of the antifungal substrates was investigated under different temperatures,and the result showed that the antifungal activity at 40 ℃ was the highest,while it decreased most at 121 ℃ and showed significant difference from the control.However,the crude protein extracts still retained 72 ％ of the antifungal activity compared to the control and the diameter of inhibition zone was 13.6 mm after heat treatment for 30 min under 121 ℃.The results above indicated that part of the antifungal extracts of Bacillus amyloliquefaciens X-278 were heat-resistant substance and the antifungal extracts were several substrates with different traits.关键词
解淀粉芽孢杆菌/抗菌蛋白/大丽轮枝菌/棉花黄萎病Key words
Bacillus amylolique faciens/antifungal protein/Verticillium dahliae/cotton Verticillium wilt分类
农业科技引用本文复制引用
黎波,魏学军,李亚宁,孟庆芳,张汀,刘大群,赤国彤..解淀粉芽孢杆菌X-278菌株抗菌蛋白初步分析[J].河南农业科学,2013,42(7):68-71,83,5.基金项目
国家863计划项目(2011AA10A205) （2011AA10A205）
国家自然科学基金项目(31171894) （31171894）
河北省自然科学基金项目(C2011204114) （C2011204114）
