河南农业科学2013，Vol.42Issue(7)：121-124,4.
CAPN1基因的多态性与鸡肉质性状的关系研究
Associations of CAPN1 Gene Polymorphism with Meat Quality Traits in Chicken
摘要
Abstract
In this study,the polymorphism of CAPN1 gene in Shouguang chicken was detected using PCR-SSCP method and its relationship with some meat quality traits of Shouguang chicken was also analyzed.At CE14 locus,six genotypes of AA,AB,AC,BB,BC,and CC were found and their frequencies were 0.238,0.273,0.197,0.095,0.102,and 0.095,respectively.Frequencies of allele A,B and C were 0.473,0.282,and 0.245,respectively.Three SNPs were revealed by sequencing,among which one was in the intron 13(g.13715467 C＞T),and two were in the exon 14 of CAPN1 gene(g.13715409 A＞G and g.13715387 T＞C).At CE20 locus,eight genotypes of AA,AB,AC,BC,AD,BB,CD,and DD were found and their frequencies were 0.122,0.354,0.197,0.143,0.061,0.034 ; 0.048,and 0.041,respectively.Frequencies of allele A,B,C,and D were 0.429,0.282,0.194,and 0.095,respectively.A total of six SNPs were identified at CE20 locus by sequencing,among which three were in the intron 19(g.13713320 A＞T,g.13713289 G＞A,and g.13713277 T＞C),one was in the exon 20(g.13713224 C＞A),and two were in the intron 20(g.13713185 T＞C and g.13713184 G＞A) of CAPN1 gene.Statistical analysis indicated that the genotypes of CE14 locus showed a significant effect on pHuB,pHuL,IMF,SFL,and CLB(P＜0.05).The genotypes of CE20 locus showed a significant effect on pHuB and CLB (P＜0.05).关键词
CAPN1基因/多态性/肉质性状/鸡/关联分析Key words
CAPN1 gene/polymorphisms/meat quality traits/chicken/association analysis分类
农业科技引用本文复制引用
周国利,郭彦,吕鑫,吴玉厚..CAPN1基因的多态性与鸡肉质性状的关系研究[J].河南农业科学,2013,42(7):121-124,4.基金项目
山东省自然科学基金项目(ZR2012CL19) （ZR2012CL19）
聊城大学博士启动基金项目(31805) （31805）
