河南农业科学2013，Vol.42Issue(7)：134-137,4.
猪伪狂犬病毒的分离与鉴定
Identification of a Swine Pseudorabies Virus Field Isolate
摘要
Abstract
An isolate of pseudorabies virus(PRV) was obtained from the tissues of dead piglets from a farm with PRV-like disease in Henan province.This isolate of PRV could induce typical cytopathogenic effects after several passages on PK-15 cells and be neutralized by PRV positive serum in microneutralization test.This isolate was sensitive to chloroform and ether,and could be inactivated in 56 ℃ water bath after 30 min.After inoculation,both rabbits and mice showed typical clinical symptoms of pseudorabies.The cloning and sequencing of gp50 gene showed that the nucleotide homology of this isolate with other 5 PRV isolates previously published in GenBank was 99 ％,indicating that this isolate is PRV.关键词
猪伪狂犬病毒/分离/鉴定Key words
porcine pseudorabies virus/isolation/identification分类
农业科技引用本文复制引用
潘霄,刘明阳,李学伍,郭成留,邓瑞广,张改平..猪伪狂犬病毒的分离与鉴定[J].河南农业科学,2013,42(7):134-137,4.基金项目
国家自然科学基金项目(31172348) （31172348）
