河南农业科学2013，Vol.42Issue(7)：145-149,5.
草鱼内脏复合蛋白酶提取工艺研究
Study on Extraction Process of Compound Protease from Viscera of Grass Carp
摘要
Abstract
In order to make full use of scraps of grass carp processing,the extraction,separation,and drying process of compound protease from viscera of grass carp were optimized with specific activity of compound protease as an index.The results showed that the best extraction condition of compound protease was pH value of 8,extraction temperature of 20 ℃,extraction time of 80 min,ultrasonic frequency of 15 kHz,ultrasonic power of 50 W,sonification time of 4 min; the best separation condition of compound protease was tea polyphenol of 8 g/L;the best drying condition of compound protease was polyethylene glycol of 20 g/L,material thickness of 6 mm,quick-freezing,drying time of 24 h.Under the conditions,the specific activity of compound protease was 4.33 U/mg.关键词
草鱼/内脏/复合蛋白酶/提取Key words
grass carp/viscera/compound protease/extraction分类
生物科学引用本文复制引用
刘明华,王大红..草鱼内脏复合蛋白酶提取工艺研究[J].河南农业科学,2013,42(7):145-149,5.基金项目
湖北省教育厅科学技术研究项目(B2013270) （B2013270）
武汉职业技术学院校级项目(2012YK671) （2012YK671）
