核农学报2013，Vol.27Issue(7)：929-937,9.
甘蓝类植物小孢子培养及植株再生研究
Microspore Embryogenesis and Plant Regeneration of Brassica Plants
摘要
Abstract
The factors that affect microspore embryogenesis and plant regeneration of 19 cultivars of Brassica plants (cabbage,broccoli and kale) were investigated in the present experiment.The results indicated that the genotype affected not only the frequency of microspore embryogenesis,but also the quality of embryos induced.Cold temperature treatment at 4℃ for 0 ～ 2 d combining with heat shock treatment at 32.5℃ for 1 d could improve embryogenesis efficiency significantly.Culturing embryos in NLN-13 liquid medium for approximately 25 d was best for embryo germination.Addition of 1％-1.2％ concentration of agar to the B5 solid differentiation medium could significantly improve microspore-derived embryo germination and plantlet regeneration.Moreover,the method that first identified the ploidy level of regenerated plantlets at early growth with flow cytometry,then soaked roots of haploids for 20 h with 200mg· L-1 colchicine solution,and finally identified with flow cytometry,could obtain doubled haploid plants quickly and efficiently.关键词
甘蓝类植物/小孢子培养/胚胎发生/植株再生/倍性鉴定Key words
Brassica plants/ Microspore culture/ Embryogenesis/ Plant regeneration / Ploidy identification引用本文复制引用
张振超,耿鑫鑫,戴忠良,潘跃平,王兵,许玲,颜志明,周伟军..甘蓝类植物小孢子培养及植株再生研究[J].核农学报,2013,27(7):929-937,9.基金项目
江苏丘陵地区镇江农科所与浙江大学合作项目(zjs2009001),国家自然科学基金(31000678),江苏省自主创新项目(cx(10)463),江苏省科技支撑计划(BE2012348),江苏省农业自主创新项目(CX(12)2004),中国博士后基金(2012T50555) （zjs2009001）
