核农学报2013，Vol.27Issue(7)：938-945,8.
水稻蜡质合成相关基因OsGL1-5的表达模式及逆境响应特征分析
Expression Pattern and Stress Responses of Wax Biosynthesis Related Gene OsGL1-5 in rice
摘要
Abstract
In this study,the promoter of wax biosynthesis related gene OsGL1-5 in rice was analyzed via bioinformatics,and the temporal and spatial expression of OsGL1-5 was analyzed by semi-quantitative RT-PCR and mRNA in situ hybridization using the different tissues and developmental stage panicles as materials.In addition,the expression of OsGL1-5 was analyzed by semi-quantitative RT-PCR when the seedlings were treated with stresses,including 200mmol· L-1 NaCl,100μmol· L-1 ABA and 1.0％ H2O2.The bioinformatics analysis showed that the promoter of OsGL1-5 (about 2 kb upstream of OsGL1-5) was predicted to contain important regulatory elements including drought,high salt,coldness,ABA,light regulation,copper,and oxygen responsive elements.The analysis of OsGL1-5 expression profiles revealed that OsGL1-5 was expressed highly in panicles at different developmental stages and specifically expressed in the trichomes of glume and the tapetum of anther,low expression of OsGL1-5 was also detected in the leaves,however,its expression was not detected in the stems and roots.The analysis of OsGL1-5 in response to stresses showed that the expression of OsGL1-5 was increased at different time when the seedlings were treated with NaCl,ABA and H2O2.关键词
水稻/OsGL1-5/表达/逆境Key words
Oryza sativa L./ OsGL1-5 / Expression / Stress引用本文复制引用
周玲艳,倪尔冬,朱丽雅,梁红,庄楚雄..水稻蜡质合成相关基因OsGL1-5的表达模式及逆境响应特征分析[J].核农学报,2013,27(7):938-945,8.基金项目
广东省自然科学基金(S2011040001653) （S2011040001653）
