华西口腔医学杂志2013，Vol.31Issue(4)：341-344,4.DOI:10.7518/hxkq.2013.04.003
牙本质基质蛋白-1仿生多肽的设计与评价
Design and evaluation of a kind of biomimetic peptides of dentin matrix protein-1
摘要
Abstract
Objective To design a kind of biomimetic polypeptide of dentin matrix protein-1 (DMP-1),which can bind to dentine collagen fibers and initiate mineralization.Methods A novel polypeptide was developed by connecting the collagen binding domain of DMP-1 “DSESSEEDR” to the hydrophilic C-terminal of amelogenin “TKREEVD”.The polypeptide was synthetically prepared by standard solid-phase peptide synthesis.Human dentine slices were completely demineralized by hydrochloric acid to expose the dentine collagen.Fluorescein isothiocyanate coupled polypeptide was applied to the exposed dentine collagen.Fluorescent microscopy was used to examine the polypeptide specially bond to the dentine collagen.Nucleation and growth of hydroxyapatite was initiated by immersing the polypeptide into calcium chloride and sodium hypophosphate solutions respectively.Scanning electron microscopy （SEM),transmission electron microscopy (TEM) and selected area diffraction (SAD) were used to examine the hydroxyapatites formed.Results Fluorescent dentine collagen was identified in the demineralized dentine specimens.Nucleation and growth of crystals were detected after immersing the polypeptide into calcium chloride and sodium hypophosphate solutions by SEM and TEM.SAD confirmed the crystals were hydroxyapatites.Conclusion The polypeptide of “DSESSEEDRTKREEVD” can simulate DMP-1 binding collagen and initiate hydroxyapatite nucleation and growth.It may be a potential molecular tool for dentine remineralization.关键词
牙本质基质蛋白-1/多肽/仿生/生物矿化Key words
dentin matrix protein-1/ polypeptide/ biomimetics/ biomineralization分类
医药卫生引用本文复制引用
刘巍,曹颖,沈军,许强建,朱振雄,李全利..牙本质基质蛋白-1仿生多肽的设计与评价[J].华西口腔医学杂志,2013,31(4):341-344,4.基金项目
国家自然科学基金资助项目(30973352):国家自然科学基金国际(地区)合作与交流项目(中国香港)(81061160511,N_HKU776/10) （30973352）
