华西口腔医学杂志2013，Vol.31Issue(4)：420-424,5.DOI:10.7518/hxkq.2013.04.022
一氧化碳对炎症环境下人牙龈成纤维细胞黏附分子表达影响的机制研究
Mechanism of carbon monoxide affecting the expression of cellular adhesion molecule under stimulation of inflammatory cytokines to human gingival fibroblasts
摘要
Abstract
[Abstract] Objective To investigate the mechanism by which carbon monoxide inhibits the expression of adhesion molecules on human gingival fibroblasts (HGF) stimulated with inflammatory cytokines.Methods HGF were cultured in vitro,and stimulated with 50ng·mL-1 tumor necrosis factor-α(TNF-α） and 10ng·mL-1 interleukin-l3 (IL-1β) concurrently in the presence or absence of carbon monoxide releasing molecule-3 (CORM-3) at 500 μmol·L-1.Expression of phosphorylated extracellular regulated protein kinase (ERK),phosphorylated c-Jun N-terminal kinase (JNK) and phosphorylated p38 in mitogen-activated protein kinase(MAPK) pathway was studied by Western blot at 10min and 20min,respectively.Nuclear expression of nuclear factor-κB (NF-κB) was checked by Western blot after 4 h stimulation.In some experiments,cells were prestimulated by 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one(ODQ) for 8 h before cytokine stimulation and the expression of intercellular adhesion molecule-1 (ICAM-1) was checked by Western blot after 24h.Results CORM-3 significantly inhibited the phosphorylation of MAPK p38 after 10min stimulation with cytokines,but had no signifi-cant effect on the phosphorylation of ERK and JNK.CORM-3 significantly inhibited the nuclear expression of NF-κB-p65 on HGF after 4 h stimulation by inflammatory cytokines.The inhibitory effect of CORM-3 on the expression of ICAM-1 was not influenced by guanylate cyclase inhibitor ODQ.Conclusion The inhibitory effect of carbon monoxide on the expression of adhesion molecules might be exerted by its inhibitory effect on the NF-κB activity and MAPK p38 phosphorylation.关键词
人牙龈成纤维细胞/核转录因子-κB/一氧化碳释放分子/丝裂原激活蛋白激酶Key words
human gingival fibroblasts/ nuclear factor-κB/ carbon monoxide releasing molecule/ mitogen-activated protein kinase分类
生物科学引用本文复制引用
赵华强,侯萌,魏玲玲,穆萍萍,宋晖,杨丕山..一氧化碳对炎症环境下人牙龈成纤维细胞黏附分子表达影响的机制研究[J].华西口腔医学杂志,2013,31(4):420-424,5.基金项目
山东省优秀中青年科学家奖励基金资助项目(BS2009S-W057) （BS2009S-W057）
山东省自然科学基金资助项目(Y2008C99) （Y2008C99）
