基础医学与临床2013，Vol.33Issue(8)：941-946,6.
人骨髓间充质干细胞体外成骨与成脂分化过程中FGF1及其受体表达的变化
The expressions of FGF1 and its receptor during the osteogenic and adipogenic differentiation of human bone marrow stromal cells
摘要
Abstract
Objective To study the dynamic expression profiles of fibroblast growth factor 1 (FGF1) and its receptors (FGFRs) during osteogenic and adipogenic differentiation of human bone marrow stromal cells (hBMSCs),and further exploring the role of FGF1 during hBMSCs differentiation.Methods Cells were isolated from human bone marrow by density gradient centrifugation method.hBMSCs in the 3rd passage were induced into osteoblasts and adipocytes respectively,and followed by staining for identification.The expressions of FGF1,FGFRs,and marker genes during osteogenic and adipogenic differentiation,were detected by real-time PCR.Immunofluorescent staining was applied for FGF1 localization of hBMSCs after induced differentiation.Meanwhile,the influence of exogenous FGF1 on osteogenic and adipogenic differentiation was detected by real-time PCR and Oil Red O stainning.Results Osteogenic and adipogenic induced hBMSCs were stained by Alizarin Red S and Oil Red O.The expression status of FGF1 and its receptors showed dynamic variation.Immunofluorescent staining results illustrated that FGF1 was mainly distributed in cytoplasm,and its expression amount increased in nucleus after osteogenic induction for 3 days.The expression of osteopontin increased during osteogenic differentiation in the presence of exogenous FGF1 for 3 days (P ＜0.05),and the amount of lipid droplet decreased and the expressions of adipogenic marker genes were suppressed during adipogenic differentiation after the introduction of exogenous FGF1 for 7 days (P ＜0.01).Conclusions FGF1 may inhibit adipogenic differentiation of hBMSCs,and promotes the expression of OPN,which may further hasten the maturity of osteogenesis.关键词
骨髓间充质干细胞/成骨分化/成脂分化/FGF1/FGFRsKey words
bone marrow mesenchymal stem cells/ osteogenic differentiation/ adipogenic differentiation/ FGF1/ FGFRs分类
生物科学引用本文复制引用
董平,焦虎,李秋晨,吕晓岩,尹艳花,肖苒..人骨髓间充质干细胞体外成骨与成脂分化过程中FGF1及其受体表达的变化[J].基础医学与临床,2013,33(8):941-946,6.基金项目
国家自然科学基金(30871433,31071305) （30871433,31071305）
