5-Aza-CdR抑制HepG2细胞DLK1基因表达及细胞生长OA北大核心CSCDCSTPCD
5-Aza-CdR inhibits DLK1 expression and cell growth of human hepatocarcinoma cell line HepG2
目的 探讨去甲基化药物5-氮杂-2’-脱氧胞苷(5-Aza-CdR)对DLK1基因及肝癌细胞系HepG2增殖、侵袭的影响.方法 不同浓度的5-Aza-CdR及PBS作用HepG2细胞后,RT-PCR、Western blot检测DLK1基因及蛋白的表达水平；MTT、Transwell和流式细胞术检测HepG2细胞的生长、侵袭力及细胞周期的变化.结果 HepG2细胞经5-Aza-CdR处理后,DLK1 mRNA、蛋白表达量降低；MTT试验显示细胞生…查看全部>>
Objective To investigate if demethylation drug 5-Aza-2'-deoxycytidine (5-Aza-CdR) may inhibit the expression of DLK1 and cell proliferation,invasion of hepatoma cell line HepG2.Methods After different concentrations of 5-Aza-CdR were applied to treat HepG2 cells,RT-PCR,Western blot were used for the detection of DLK1 mRNA and protein level change; MTT,Transwell and flow cytometry were used for detection of cell growth,cycle and invasiveness.Results After tre…查看全部>>
黄铀新;罗耀玲;刘瑶
赣南医学院第一附属医院核医学科,江西赣州341000赣南医学院第一附属医院临床医学研究中心,江西赣州341000赣南医学院第一附属医院消化内科,江西赣州341000
医药卫生
HepG2细胞5-Aza-CdRDLK1基因
HepG2 cell 5-Aza-CdR DLK1
《基础医学与临床》 2013 (8)
肝癌中PEG10基因印记状态改变的表观遗传学机制研究
966-970,5
国家自然科学基金(81000908)
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