基础医学与临床2013，Vol.33Issue(8)：966-970,5.
5-Aza-CdR抑制HepG2细胞DLK1基因表达及细胞生长
5-Aza-CdR inhibits DLK1 expression and cell growth of human hepatocarcinoma cell line HepG2
摘要
Abstract
Objective To investigate if demethylation drug 5-Aza-2'-deoxycytidine (5-Aza-CdR) may inhibit the expression of DLK1 and cell proliferation,invasion of hepatoma cell line HepG2.Methods After different concentrations of 5-Aza-CdR were applied to treat HepG2 cells,RT-PCR,Western blot were used for the detection of DLK1 mRNA and protein level change; MTT,Transwell and flow cytometry were used for detection of cell growth,cycle and invasiveness.Results After treatment with 5 μmol/L 5-Aza-CdR,the expression of DLK1 mRNA and protein decreased,the cell growth rate was slowed,the number of G1 phase cells decreased where the S phase cells increased,Transwell confirmed invasive ability decreased significantly (P ＜ 0.05).Conclusions 5-Aza-CdR can effectively inhibit DLK1 gene expression,thereby inhibiting tumor cell growth,proliferation,invasion of HepG2.关键词
HepG2细胞/5-Aza-CdR/DLK1基因Key words
HepG2 cell/ 5-Aza-CdR / DLK1分类
医药卫生引用本文复制引用
黄铀新,罗耀玲,刘瑶..5-Aza-CdR抑制HepG2细胞DLK1基因表达及细胞生长[J].基础医学与临床,2013,33(8):966-970,5.基金项目
国家自然科学基金(81000908) （81000908）
