基础医学与临床2013，Vol.33Issue(8)：981-985,5.
靶向COL1A1基因的shRNA对乳腺癌MDA-MB-231细胞体外侵袭与迁移的影响
Effects of shRNA targeting COL1A1 gene on the invasion and migration of human breast cancer MDA-MB-231 cell line in vitro
摘要
Abstract
Objective To investigate the effects of shRNA-mediated human collagen type 1 alpha 1 (COL1A1)gene silence on the migration and invasion of Breast Cancer Cell Line MDA-MB-231.Methods Two shRNA vectors and one negative control vector were designed and stably transfected into MDA-MB-231 cells.Western blot analysis was used to screen for the group which had the highest inhibitory rate.Monolayer colony formation assay was performed to assess a single cell proliferation ability.The cell adhesion,migration and invasion potencies were observed by cell-matrix adhesion assay,and Transwell assay.Results Cells transfected with pshRNA-COL1A1-2 vector had the stronger inhibition of COL1A1 protein,with the inhibition rate being 66.98％ ± 2.08％,and cells in this group were used for the subsequent experiments.Compared with control group and pshRNA-scramble group,the monolayer colony formation rate decreased obviously (P ＜ 0.05).The optical absorbance value of adherent cells and the number of invading and migrating cells which moved across the matrix barrier for pshRNA-COL1A1 group were less than that of control group and pshRNA-scramble group(P ＜ 0.001).Conclusions COL1A1 gene silencing of human MDA-MB-231 cells may inhibit cell matrix adhesion,migration and invasion potencies in vitro.关键词
shRNA/COL1A1/乳腺癌/侵袭/迁移Key words
shRNA/ COL1A1/ breast cancer/ invasion/ migration分类
医药卫生引用本文复制引用
余海浪,马文丽,周珏宇,孟伟,石嵘,郑文岭..靶向COL1A1基因的shRNA对乳腺癌MDA-MB-231细胞体外侵袭与迁移的影响[J].基础医学与临床,2013,33(8):981-985,5.基金项目
广东省医学科研基金(B2012203) （B2012203）
广东省领军人才专项基金(C1030925) （C1030925）
