基础医学与临床2013，Vol.33Issue(8)：992-997,6.
力达霉素在体内外抑制小鼠骨髓瘤细胞系SP2/0移植成瘤
Lidamycin inhibits mouse myeloma SP2/0 in vivo and in vitro
摘要
Abstract
Objective To investigate the effect of Lidamycin (LDM) against mouse myeloma in vivo and in vitro and provide the basis for its use in therapy.Methods MTS assay was used to detect SP2/0 cell proliferation.Cell cycle distribution and cell apoptosis were measured by flow cytometry combined with propidium iodide (PI) staining.The expression of proteins was determined by Western blot analysis.in vivo antitumor activity was carried out using a mufine myeloma model in BALB/c mice.Results There was a significant reduction of proliferation in the cells treated with LDM.The overall growth inhibition correlated with apoptotic cell death.LDM induced cell apoptosis was associated with decrease of NF-κB,BCL-2,and Survivin.LDM induced G2/M phase arrest through increase the expression of p27 protein in SP2/0 cells.LDM markedly suppressed tumor growth in murine myeloma model.Conclusions LDM demonstrates a significant antitumor efficacy against myeloma SP2/0 in vivo and in vitro.关键词
力达霉素/细胞凋亡/鼠骨髓瘤/抗肿瘤Key words
lidamycin/ cell apoptosis/ mouse myelom/ anti-tumor分类
医药卫生引用本文复制引用
甄永占,赵毓芳,骆广玲,章广玲,刘学军,李冉,阚泉..力达霉素在体内外抑制小鼠骨髓瘤细胞系SP2/0移植成瘤[J].基础医学与临床,2013,33(8):992-997,6.基金项目
河北省自然科学基金(H2012401030) （H2012401030）
