基础医学与临床2013，Vol.33Issue(8)：1009-1013,5.
高浓度葡萄糖通过下调自噬增加体外培养雪旺细胞的凋亡
High concentration of glucose increases apoptosis of Schwann cell in vitro by downregulation of autophagy
摘要
Abstract
Objective To observe the effect of the high concentration of glucose on autophagy of rat's Schwann cells in vitro and the effect of autophagy on the proliferation and apoptosis of Schwann cells cultured in high glucose medium.Methods RSC96 cells were cultured.The expression of beclinl and caspase-3 were detected by immunofluorescence and Western blot.The proliferation was detected by MTT.Results The expression of beclin1 in RSC96 cells cultured in high glucose medium was reduced (P ＜ 0.05),as well as the proliferation was decreased (P ＜0.05,P ＜0.01).Inhibition of autophagy resulted in aggravating the decrease of the proliferation of RSC96 cells cultured in high glucose medium (P ＜ 0.01) and upregulating the activity of caspase-3 (P ＜ 0.01).Conclusions The impairment of the high concentration of glucose increased apoptosis by downregulation of autophagy.关键词
雪旺细胞/高糖/自噬/beclin1/凋亡Key words
schwann cells/ high glucose/ autophagy/ beclin1 / apoptosis分类
医药卫生引用本文复制引用
屈岭,顾蓓,张宏,石玥,梁晓春..高浓度葡萄糖通过下调自噬增加体外培养雪旺细胞的凋亡[J].基础医学与临床,2013,33(8):1009-1013,5.基金项目
北京协和医院青年科研基金(2009) （2009）
