基础医学与临床2013，Vol.33Issue(8)：1019-1023,5.
荧光显微镜观察封闭式脊髓窗内的大鼠脊髓微循环
Observation of the spinal microcirculation in rat through a closed spinal window by fluorescence microscopy
摘要
Abstract
Objective To investigate the feasibility and efficacy of closed spinal window applied to assess the rat dorsal pial microcirculation by fluorescence microscopy.Methods On the 8-week-old SD rats,we made a precise improved closed spinal window with a laminectomy at the T10 level using a dental acrylic resin and a cover glass.The window was placed over the back of the rat with intact dura.Pasted the dental acrylic resin onto the spinous processes at the T9 and T11 as fixed aids.Collect 100 μL hematocrit red blood cells (BRC) by centrifugation (2 000 ×g).The RBC labelled by Dil (5 mg/L) in vitro were injected in jugular vein.Leukocytes labelled with rhodamine 6G were injected intravenously(0.3 g/L).The spine was fixed with vertebral fixation device.Local microcirculation is recorded in real time through the window.Results Microvascular morphology,distribution and blood vessel density can be observed.Dil-labeled RBC velocity and adhesion were observed.Leukocyte adherence to pial vessels and assessment of perfusion were observed with the help of rhodamine 6G.Conclusions By improved closed spinal window and vertebral fixation device,we observed the microcirculation in the rat pia.关键词
大鼠/脊髓/微循环/活体荧光/脊髓窗Key words
rat / spinal/ microcirculation / intravital fluorescence / spinal window分类
医药卫生引用本文复制引用
苑晓晨,李炳蔚,武清斌,荆瀛黎,盛有明,李宏伟,刘淑英,修瑞娟..荧光显微镜观察封闭式脊髓窗内的大鼠脊髓微循环[J].基础医学与临床,2013,33(8):1019-1023,5.基金项目
2011年中国医学科学院微循环研究所公益性科研院所基本科研业务费专项基金 （）
