解放军医学杂志2013，Vol.38Issue(8)：670-674,5.
黑火药烟雾致吸入性肺损伤大鼠模型的建立与评价
Reproduction and evaluation of a rat model of inhalation lung injury caused by black gunpowder smog
摘要
Abstract
Objective To reproduce and evaluate a rat model of inhalation lung injury caused by black gunpowder smog.Methods The smog composition was analyzed and a rat model of inhalation lung injury was reproduced.Forty two healthy male Wistar rats were randomly divided into normal control (NC) group and 1h,2h,6h,24h,48h and 96h after inhalation group (n=6).The arterial blood gas,wet to dry weight ratio (W/D) of lung,leukocyte count,and protein concentration in broncho-alveolar lavage fluid (BALF) were determined.Macroscopic and microscopic changes in lung tissue were observed.Results The composition of black gunpowder smog was composed mainly of CO2 and CO,and their concentrations remained stable within 12 minutes.Smog inhalation caused a significant hypoxemia,the concentration of blood COHb reached a peak value 1h,and the W/D of lung reached peak value 2h after inhalation (P＜0.05).The amount of leukocytes and content of protein in BALF increased significantly within 24h after inhalation (P＜0.05).Histopathological observation showed diffuse hemorrhage,edema and inflammatory cell infiltration in lung tissue as manifestations of acute lung injury,and the injury did not recover at 96h after inhalation.Conclusion The rat model of inhalation lung injury can be reproduced using black gunpowder smog,and it has the advantages of its readiness for reproduction,reliability and stability,and it could be used for the experiment of inhalation injury in a battlefield environment.关键词
黑火药/烟雾吸入损伤/模型,动物Key words
black powder/ smoke inhalation injury/ models, animal分类
医药卫生引用本文复制引用
刘一凡,唐红卫,吴小利,谢尹晶,张洪瑞,段晋燕,向代军,兰晓梅,李绵洋..黑火药烟雾致吸入性肺损伤大鼠模型的建立与评价[J].解放军医学杂志,2013,38(8):670-674,5.基金项目
全军"十二五"医学科研基金面上项目(CWS12J021) （CWS12J021）
This work was supported by the "Twelfth Five-Year Plan" Medical Science Development Foundation of PLA (CWSI2J021) （CWSI2J021）
