吉林农业大学学报2013，Vol.35Issue(4)：416-419,4.
木耳emg1基因克隆与生物信息学分析
Cloning and Bioinformatics Analysis of emg1 Gene of Auricularia auricular-judae
摘要
Abstract
PCR products Aa-2 were amplified with degenerate primers designed according to the cDNA sequences of Auricularia nigricans and Auricularia delicata with the length of 1 075 bp.The E value of Aa-2 is 0 by blastn with A.delicata genome sequence of JGI databank and 1.82e-112 by blastx with Auricularia delicata emg 1 gene of JGI databank.775 bp are identical between the Aa-2 sequence and the Auricularia auricularia-judae emg1 gene,among which the genome was sequenced by our lab.The homology of part Aa-2 fragments to emg1 cDNA fragments of Sporisorium reilianum is 91％ by blastn.The E value of Aa-2 fragments is 2.66e-43 compared with the emg1 superfamily protein sequence.The Aa-2 fragment was preliminarily identified as emg1 CDS.关键词
木耳/emg1/基因克隆Key words
Auricularia auricula-judae / emg 1 / gene cloning分类
农业科技引用本文复制引用
方明,姚方杰,王晓娥,陈影..木耳emg1基因克隆与生物信息学分析[J].吉林农业大学学报,2013,35(4):416-419,4.基金项目
现代农业产业技术体系专项 （）
