环境与职业医学2013，Vol.30Issue(7)：520-523,4.
煤烘高氟玉米染毒大鼠的血清TRACP-5b表达变化
Expression Variation of Serum TRACP-5b in Rats Treated with Coal-Burning Fluorine
摘要
Abstract
[Objective] To observe the expression variation of serum tartrate-resistant acid phosphatase isoform 5b (TRACP-5b)in rats exposed to coal-burning fluorine,and explore the role of bone resorption in the pathogenesis of skeletal fluorosis.[Methods] One hundred and twenty SD rats weaned for 4 weeks were divided into 4 groups:1 control group was fed with commercial feed,and the other 3 experimental groups were fed with mixed feeds which contained 9.56 mg/kg (low-dose group),15.89 mg/kg (mediumdose group),and 23.00mg/kg (high-dose group) of fluorine.The animals were executed after 30d,90d,and 180d of treatment to determine bone and urinary fluoride content,incidence rate of dental fluorosis,and femur pathological score.TRACP-5b levels in serum were tested by ELISA.[Results] (1) The bone and urinary fluoride concentrations,the dental fluorosis incidences,and the femur pathological scores of the 3 experimental groups were significantly increased in a dose-dependent manner respectively (all P＜ 0.05).After the treatment for 30d,60d,and 90d,the urinary and bone fluoride concentrations of each experimental group were statistically higher than the control group (all P＜ 0.05),and those of the higher dose group were remarkably higher than those of the lower dose group respectively (all P ＜ 0.05).For 90 d and 180 d,the incidence of dental fluorosis in each dose group was obviously higher than that of controls (all P＜ 0.05); the femur pathological score of the medium-dose and the high-dose groups were significantly higher than that of the low-dose group and the controls (all P＜ 0.05).(2) The serum TRACP-5b levels in each experimental group with 180d of exposure were statistically higher than those in the same dose group with 30d and 90d of exposure (all P＜0.05).There was no significant difference in the serum TRACP-5b among the groups after treatment for 30d and 90d.After the treatment for 180d,the TRACP-5b levels of the high-dose group was significantly higher than that of the controls (all P＜0.05),and no significantly differences were found among the other groups.[Conclusion] Exposure to high doses of fluorine for a longterm basis may cause elevated expression of serum TRACP-5b.关键词
燃煤型氟中毒/抗酒石酸酸性磷酸酶5b/大鼠Key words
coal-burning fluorosis/ tartrate-resistant acid phosphatase isoform 5b/ rat分类
医药卫生引用本文复制引用
韦艳,张震,刘新儒,张华..煤烘高氟玉米染毒大鼠的血清TRACP-5b表达变化[J].环境与职业医学,2013,30(7):520-523,4.基金项目
科技部国际合作项目(编号:2010DFB30530) （编号:2010DFB30530）
贵州省科学技术基金(编号:黔科合J字[2010]2268号) （编号:黔科合J字[2010]2268号）
