环境与职业医学2013，Vol.30Issue(7)：551-554,4.
用HOPE-MED8050动式染尘控制系统建立大鼠矽肺模型
Rats Silicosis Model with Dynamic Inhalation Exposure to Silica Dust by HOPE-MED8050 System
摘要
Abstract
[Objective] To establish a rat silicosis model by a dynamic silica dust exposure control system.[Methods] Forty SD rats were randomly divided into control group,and 1 d,3 d,7d,2-week,4-week,8-week,and 12-week groups,5 rats for each group.The rats were exposed to silica dust for 2h a day in a dynamic control system at the concentration of 170-190mg/m3.After the exposure,the animals were sacrificed according to the assigned group and the control group rats did after 12weeks.The body weight and lung weight were recorded to calculate lung/body coefficients.Hydroxy proline (Hyp) in the lung tissue was detected and pathological changes were observed.[Results] The concentration of silica dust in the exposure chamber was maintained stable.The lung coefficient gradually increased with the time of exposure.Mild congestion and edema were observed in the lung tissue in the 1 d group; inflammatory cells around blood vessels were recorded in the 7 d group,most of them were monocytes and lymphocytes.In the 2-week group,red dye and foam cells were found in the alveoli.In the 4-week group,droppod-off alveolar epithelial cells,phagocytes-formed nodules,and a small amount of fibrous tissue hyperplasia were identified.In the 8-week group,more fibrous hyperplasia were observed,plus the gland-like hyperplasia of type Ⅱ alveolar epithelial cells.Fibrous nodules appeared in the 12-week group,and no abnormality in lung was identified in the control group.The level of Hyp in the lung tissue increased gradually along with the exposure duration to silica dust.[Conclusion] The rats silicosis model could be well established by the dynamic control system.关键词
矽肺/动物模型/动式染毒Key words
silicosis/ animal model/ dynamic exposure分类
医药卫生引用本文复制引用
王晔,杨茜,程薇波,梁锐超,薛灵抒,曹莹莹..用HOPE-MED8050动式染尘控制系统建立大鼠矽肺模型[J].环境与职业医学,2013,30(7):551-554,4.基金项目
教育部博士点基金(新教师)(编号:20100181120096) （新教师）
四川省基础研究项目(编号:2011JY0019) （编号:2011JY0019）
